Ache^tm1.1Hssh
Targeted Allele Detail

Summary

• Symbol: Ache^tm1.1Hssh
• Name: acetylcholinesterase; targeted mutation 1.1, Hee-Sup Shin
• MGI ID: MGI:3814333
• Gene: Ache Location: Chr5:137286516-137292728 bp, + strand Genetic Position: Chr5, 76.32 cM
• Alliance: Ache^tm1.1Hssh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140622
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Transient cre expression in ES cells was used to remove the neo cassette and exon 3. The absence of protein expression was confirmed by northern blot analysis and enzymatic activity assay on brain extracts. (J:140622)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ache Mutation: 30 strains or lines available

References

• Original: J:140622 Sun M, et al., Reduced nicotinic receptor function in sympathetic ganglia is responsible for the hypothermia in the acetylcholinesterase knockout mouse. J Physiol. 2007 Feb 1;578(Pt 3):751-64
• All: 1 reference(s)