Trpa1^tm2.1Kykw
Targeted Allele Detail

Summary

• Symbol: Trpa1^tm2.1Kykw
• Name: transient receptor potential cation channel, subfamily A, member 1; targeted mutation 2.1, Kelvin Y Kwan
• MGI ID: MGI:3814429
• Synonyms: Trpa1^loxP
• Gene: Trpa1 Location: Chr1:14942872-14989086 bp, - strand Genetic Position: Chr1, 4.5 cM
• Alliance: Trpa1^tm2.1Kykw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169738
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were placed on either side of the exons that encode for the S5/S6 transmembrane domains of the gene followed by an FRT-flanked self-excising neomycin resistance cassette placed in the opposite transcriptional orientation. The neomycin cassette was excised through a cross with a Gt(ROSA)26Sor FLP strain on an unknown genetic background and the floxed exons were deleted through a cross with a Cre strain with a widespread pattern of expression on an unknown genetic background. Cre has been selectively bred away from the line. (J:169738)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpa1 Mutation: 94 strains or lines available

References

• Original: J:169738 Corey D, et al., Generation of a conditional allele of Trpa1. MGI Direct Data Submission. 2011
• All: 5 reference(s)