Gt(ROSA)26Sor^{tm1(PDGFRA*)Hsc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(PDGFRA*)Hsc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Hubert Schorle
• MGI ID: MGI:3814559
• Synonyms: hPDGFRalpha D842V, R26^hPDGFRaPM, ROSA26 knock in human PDGFRalpha D842V
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(PDGFRA*)Hsc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145520
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv x C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(PDGFRA*)Hsc} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PDGFRA (5156)

  Pdgfra

  (MGI:97530)

• Mutation details: The Gt(ROSA)26Sor gene is interrupted by a DNA sequence encoding a constitutively active mutant human platelet derived growth factor receptor, alpha polypeptide, in which the aspartic acid at amino acid position 842 has been replaced by valine (D842V). The PDGFRA sequence is preceded by a loxP flanked cassette containing termination codons in all three reading frames. In the presence of cre recombinase, this STOP cassette is excised, permitting expression of the mutant receptor from the native Gt(ROSA)26Sor promoter. (J:145520)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

ES cell line = Knut1.

References

• Original: J:145520 Moenning A, et al., Sustained platelet-derived growth factor receptor alpha signaling in osteoblasts results in craniosynostosis by overactivating the phospholipase C-gamma pathway. Mol Cell Biol. 2009 Feb;29(3):881-91
• All: 3 reference(s)