Cthrc1^tm1Hssk
Targeted Allele Detail

Summary

• Symbol: Cthrc1^tm1Hssk
• Name: collagen triple helix repeat containing 1; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:3815067
• Gene: Cthrc1 Location: Chr15:38940327-38950516 bp, + strand Genetic Position: Chr15, 15.35 cM, cytoband C
• Alliance: Cthrc1^tm1Hssk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140886
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: EB3 ES cells were targeted with a vector containing a lacZ and PGK-neo cassette, removing exon 1, including the translation initiation codon. Absence of transcript was confirmed by Northern blot analysis. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. (J:140886)

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cthrc1 Mutation: 34 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:140886 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:140886 Yamamoto S, et al., Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex. Dev Cell. 2008 Jul;15(1):23-36
• All: 4 reference(s)