Nr1i3^{tm1(NR1I3)Arte}
Targeted Allele Detail

Summary

• Symbol: Nr1i3^{tm1(NR1I3)Arte}
• Name: nuclear receptor subfamily 1, group I, member 3; targeted mutation 1, TaconicArtemis
• MGI ID: MGI:3815103
• Synonyms: huCAR
• Gene: Nr1i3 Location: Chr1:171041503-171046414 bp, + strand Genetic Position: Chr1, 79.21 cM
• Alliance: Nr1i3^{tm1(NR1I3)Arte} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140975
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 9 were replaced with the human gene (exons 2 through 9 with intronic sequences) with an attB53 site upstream of the human exon 3 and an frt-flanked neo cassette with a 3' attP50 following the human exon 9. ES cells originally used contained Nr1i2^{tm1(NR1I2)Arte}. Germ line, flp mediated recombination was used to remove the neo cassette. The absence of the endogenous transcript expression was confirmed by qPCR on liver and small intestine extracts. (J:140975)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Nr1i3 Mutation: 36 strains or lines available

References

• Original: J:140975 Scheer N, et al., A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J Clin Invest. 2008 Sep;118(9):3228-39
• All: 12 reference(s)