Cidec^tm1Maka
Targeted Allele Detail

Summary

• Symbol: Cidec^tm1Maka
• Name: cell death-inducing DFFA-like effector c; targeted mutation 1, Masato Kasuga
• MGI ID: MGI:3815158
• Synonyms: Fsp27^-
• Gene: Cidec Location: Chr6:113401595-113412721 bp, - strand Genetic Position: Chr6, 52.76 cM, cytoband E3
• Alliance: Cidec^tm1Maka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140863
• Parent Cell Line: EB3/5 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on white adipose tissue extracts. ES cells used to create this allele were EB3/5. (J:140863)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cidec Mutation: 15 strains or lines available

Notes

ES cell line EB3/5 was derived from ES cell line E14TG2a. EB3/5 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:140863 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:140863 Nishino N, et al., FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets. J Clin Invest. 2008 Aug;118(8):2808-21
• All: 2 reference(s)