Hprt1^{tm1(Nfkb1-EGFP)Cjo}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Nfkb1-EGFP)Cjo}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Christian Jobin
• MGI ID: MGI:3815189
• Synonyms: cis-NF-kappaB^EGFP, NF-kappaB^EGFP, NFkappaB^EGFP
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Nfkb1-EGFP)Cjo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92020
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Three NF-kappa B response elements derived from the 5' LTR of the HIV genome and a minimal c-fos promoter were placed upstream of a EGFP reporter gene. This reporter construct was inserted upstream of genomic sequence containing the promoter and first two exons of the Hprt gene locus that is deleted in BK ES cells. Correct recombination rescued the Hprt defect, ensuring that only a single copy of the NF-kappaB reporter construct integrated into the Hprt locus. GFP positive mononuclear cells are found in both male and female mice. (J:92020)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:92020 Magness ST, et al., In vivo pattern of lipopolysaccharide and anti-CD3-induced NF-kappa B activation using a novel gene-targeted enhanced GFP reporter gene mouse. J Immunol. 2004 Aug 1;173(3):1561-70
• All: 18 reference(s)