Cbfa2t3^tm1.1Swh
Targeted Allele Detail

Summary

• Symbol: Cbfa2t3^tm1.1Swh
• Name: CBFA2/RUNX1 translocation partner 3; targeted mutation 1.1, Scott W Hiebert
• MGI ID: MGI:3815201
• Synonyms: Mtg16^-
• Gene: Cbfa2t3 Location: Chr8:123351880-123425848 bp, - strand Genetic Position: Chr8, 71.93 cM, cytoband E1
• Alliance: Cbfa2t3^tm1.1Swh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140967
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cbfa2t3^tm1Swh heterozygotes were crossed with transgenic cre mice resulting in excision of exon 8 from the germline. It was predicted to be a null allele because splicing from exon 7 to exon 9 introduces a stop codon and would trigger nonsense-mediated mRNA decay. Gene inactivation in homozygotes was confirmed by a lack of signal in immunoblot assays of splenocyte nuclear extracts. (J:140967)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cbfa2t3^tm1.1Swh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbfa2t3 Mutation: 34 strains or lines available

References

• Original: J:140967 Chyla BJ, et al., Deletion of Mtg16, a target of t(16;21), alters hematopoietic progenitor cell proliferation and lineage allocation. Mol Cell Biol. 2008 Oct;28(20):6234-47
• All: 13 reference(s)