Tg(Pcna*K164R)1Mdsc
Transgene Detail

Summary

• Symbol: Tg(Pcna*K164R)1Mdsc
• Name: transgene insertion 1, Matthew D Scharff
• MGI ID: MGI:3817318
• Synonyms: Pcna^K164R
• Transgene: Tg(Pcna*K164R)1Mdsc Location: unknown
• Alliance: Tg(Pcna*K164R)1Mdsc page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pcna*K164R)1Mdsc expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pcna (MGI:97503)

• Mutation details: A genomic fragment was generated by SphI digestion that contained about 3 kb of the 5' promoter region of the murine Pcna gene, all of its exon and intron sequences, and 875 bp of its 3'untranslated region. An A to G mutation was introduced by site-directed mutagenesis to change codon 164 from lysine to arginine (AGG) and to generate a new EcoNI restriction site. Real-time PCR on B cell extracts showed an approximately two- to eight- fold increase of steady-state mRNA compared to mRNA levels derived from the endogenous Pcna locus. Immunoblot assays on B cell extracts confirmed higher expression of the transgene. The point mutation is predicted to interfere with ubiquitylation of the protein product. (J:141098)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:141098 Roa S, et al., Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16248-53
• All: 1 reference(s)