Tg(Nes-cre/ERT2)4Imayo
Transgene Detail

Summary

• Symbol: Tg(Nes-cre/ERT2)4Imayo
• Name: transgene insertion 4, Itaru Imayoshi
• MGI ID: MGI:3817325
• Synonyms: Nes-CreER^T2, Nestin-CreER^T2line4, Tg(Nes-cre/ERT2)4Kag
• Transgene: Tg(Nes-cre/ERT2)4Imayo Location: unknown
• Alliance: Tg(Nes-cre/ERT2)4Imayo page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Nes-cre/ERT2)4Imayo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Nes (25491)

  Nes	(MGI:101784)

• Mutation details: This transgene consists of a 5.8 kb fragment of the rat Nes promoter and a 1.8 kb fragment of the second intron of Nes driving expression of a tamoxifen-inducible form of cre recombinase followed by an SV40 poly A sequence. Three lines were created (lines 1, 4, and 5-1) with differing levels of activity (high, medium, and low, respectively) and leakiness (high, low and none, respectively). (J:110145)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (rat) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities Alliance rat Nes gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Eno2^tm1(DTA)Hsak/Eno2^tm1(DTA)Hsak Tg(Nes-cre/ERT2)4Imayo/0	involves: C57BL/6
tg2	Tg(Nes-cre/ERT2)4Imayo/0	involves: C57BL/6

Phenotypes:

Affected Systems	cn1	tg2
show or hide all annotated terms
behavior/neurological	√
behavior/neurological phenotype	N
abnormal contextual conditioning behavior	√
abnormal long-term spatial reference memory	√
cellular		√
decreased neuronal precursor proliferation		√
nervous system	√	√
decreased neuronal precursor proliferation		√
hydrocephaly		√
decreased brain size		√
abnormal dentate gyrus morphology	√
abnormal olfactory bulb morphology	√
decreased neuron number	√
decreased cerebellar granule cell number	√
reproductive system		√
increased miscarriage rate		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:110145 Imayoshi I, et al., Temporal regulation of Cre recombinase activity in neural stem cells. Genesis. 2006 May;44(5):233-8
• All: 27 reference(s)