Zbtb7b^tm2Itan
Targeted Allele Detail

Summary

• Symbol: Zbtb7b^tm2Itan
• Name: zinc finger and BTB domain containing 7B; targeted mutation 2, Ichiro Taniuchi
• MGI ID: MGI:3817518
• Synonyms: ThPok^GFP:PEdelta
• Gene: Zbtb7b Location: Chr3:89284953-89300976 bp, - strand Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
• Alliance: Zbtb7b^tm2Itan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141142
• Parent Cell Line: M1/M-1 (ES Cell)
• Strain of Origin: (129 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: ES cells containing the GFP knock-in allele, Zbtb7b^tm1Tani, were targeted for a second mutation. A loxP site was placed approximately 6 kb upstream of the transcription start site, placing it 5' to a proximal enhancer for this gene locus. A floxed neo-cassette was placed downstream of the enhancer. Transient expression of cre-recombinase removed a 2.2-kb region including the proximal enhancer and the neo cassette. Southern blotting confirmed correct targeting of the knock in allele. (J:141142)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zbtb7b Mutation: 26 strains or lines available

Notes

Mutant ES cell line = M1 heterozygous for the Zbtb7b^tm1Tani allele

References

• Original: J:141142 Muroi S, et al., Cascading suppression of transcriptional silencers by ThPOK seals helper T cell fate. Nat Immunol. 2008 Oct;9(10):1113-21
• All: 5 reference(s)