Hsd11b1^tm1.1Ggla
Targeted Allele Detail

Summary

• Symbol: Hsd11b1^tm1.1Ggla
• Name: hydroxysteroid 11-beta dehydrogenase 1; targeted mutation 1.1, Gareth Lavery
• MGI ID: MGI:3817966
• Gene: Hsd11b1 Location: Chr1:192903917-192946345 bp, - strand Genetic Position: Chr1, 97.67 cM
• Alliance: Hsd11b1^tm1.1Ggla page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141273
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the neo cassette and exon 5. RT-PCR confirmed the absence of transcript expression in the liver, tibialis anterior amd soleus. The absence of enzymatic activity was confirmed. (J:189132)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsd11b1 Mutation: 28 strains or lines available

References

• Original: J:141273 Swali A, et al., 11beta-Hydroxysteroid dehydrogenase type 1 regulates insulin and glucagon secretion in pancreatic islets. Diabetologia. 2008 Nov;51(11):2003-11
• All: 18 reference(s)