Cdk1^{tm1(Cdk2)Kald}
Targeted Allele Detail

Summary

• Symbol: Cdk1^{tm1(Cdk2)Kald}
• Name: cyclin dependent kinase 1; targeted mutation 1, Philipp Kaldis
• MGI ID: MGI:3818306
• Synonyms: Cdk1^{Cdk2delta12-HA}, Cdk1^Cdk2KI, Cdk1^{Cdk2N3DF4YQ5IV7I-HA}
• Gene: Cdk1 Location: Chr10:69170976-69188742 bp, - strand Genetic Position: Chr10, 36.07 cM
• Alliance: Cdk1^{tm1(Cdk2)Kald} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141249
• Parent Cell Line: S1B6 (ES Cell)
• Strain of Origin: 129S1/SvImJ or C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Cdk1^{tm1(Cdk2)Kald} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cdk2 (MGI:104772)

• Mutation details: A construct containing an upstream FRT site, coding sequence for the Cdk2 protein that excludes the first 12 amino acids and includes a carboxy-terminus HA tag, an IRES-beta-galactosidase gene, another FRT side and a loxP-flanked neomycin gene was used to target exon 3 of the gene locus. The insertion site maintains all the exon 3 splicing sequences of the endogenous locus. This targeted mutation resulted in a transcript that fused the 5'UTR encoded by exon 1 and the ATG start codon plus 11 amino acids encoded by exon 2 to the coding sequence of the inserted gene. The first 12 amino acids of the endogenous gene and the knock-in are very similar (they differ by four amino acids- N3D, F4Y, Q5I and V7I), suggesting that this region would not affect the Cdk2 knock-in properties. Lysates of mouse embryonic fibroblasts isolated from heterozygote mice indicated that the knock-in Cdk2 was expressed and displayed kinase activity similar to that of endogenous Cdk2. In addition, extensive Beta-galactosidase staining was observed in the spermatocytes of heterozygous mice. (J:141249)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk1 Mutation: 21 strains or lines available

References

• Original: J:141249 Satyanarayana A, et al., Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. Development. 2008 Oct;135(20):3389-400
• All: 1 reference(s)