Tgfbr3^tm1.1Jba
Targeted Allele Detail

Summary

• Symbol: Tgfbr3^tm1.1Jba
• Name: transforming growth factor, beta receptor III; targeted mutation 1.1, Joey V Barnett
• MGI ID: MGI:3818438
• Gene: Tgfbr3 Location: Chr5:107254436-107437495 bp, - strand Genetic Position: Chr5, 51.9 cM
• Alliance: Tgfbr3^tm1.1Jba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141344
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 of this locus encodes the N terminus, including a portion of the extracellular ligand binding domain. A floxed neo-cassette was placed upstream of exon 3 and a single loxP site was placed downstream of exon 3. Mice carrying the targeted mutation in the germline were crossed with cre-transgenic mice to remove exon 3 and the neo-cassette, leaving in its place a single loxP site. Ligand crosslinking with ¹²⁵I-TGFbeta to embryonic fibroblasts isolated from homozygotes confirms loss of protein expression. (J:141344)

Expression

In Mice Carrying this Mutation:	86 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tgfbr3 Mutation: 52 strains or lines available

References

• Original: J:141344 Compton LA, et al., Coronary vessel development is dependent on the type III transforming growth factor beta receptor. Circ Res. 2007 Oct 12;101(8):784-91
• All: 3 reference(s)