Zbtb7b^tm2Litt
Targeted Allele Detail

Summary

• Symbol: Zbtb7b^tm2Litt
• Name: zinc finger and BTB domain containing 7B; targeted mutation 2, Dan R Littman
• MGI ID: MGI:3818473
• Synonyms: ThPOK-GFP
• Gene: Zbtb7b Location: Chr3:89284953-89300976 bp, - strand Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
• Alliance: Zbtb7b^tm2Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141519
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 were replaced with enhanced green fluorescent protein (EGFP) coding sequence followed by the SV40 polyadenlyation signal. During the gene targeting, a loxP-flanked PGK-neomycin cassette was inserted approximately 1.1 kb upstream of the initiation codon in the sense orientation. The PGK-neomycin cassette was deleted in vivo by crossing to EIIa-cre mice and a single loxP site remains. In T lymphocytes of heterozygotes, GFP is specifically expressed in CD4 cells. In homozygotes, B220+ cells also express GFP. (J:141144, J:141519)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zbtb7b Mutation: 26 strains or lines available

References

• Original: J:141519 Littman DR, Generation of a Zbtb7b GFP knock-in allele. MGI Direct Data Submission. 2008
• All: 11 reference(s)