Runx3^tm1Litt
Targeted Allele Detail

Summary

• Symbol: Runx3^tm1Litt
• Name: runt related transcription factor 3; targeted mutation 1, Dan R Littman
• MGI ID: MGI:3818488
• Synonyms: Runx3-dYFP, Runx3d-YFP, Runx3^tm1(YFP)Litt
• Gene: Runx3 Location: Chr4:134847963-134905301 bp, + strand Genetic Position: Chr4, 67.19 cM
• Alliance: Runx3^tm1Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141144
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Yellow fluorescent protein attached to the palmitoylation domain of neuromodulin (YFP-Mem) was inserted into the first coding region of the targeted gene and its expression was driven by the Runx3 distal promoter. A PGK-neomycin resistance cassette flanked by loxP sites was placed within the first exon immediately 3' of YFP. Protein expression of the targeted gene was almost undetectable in CD8⁺ T cells and was much lower in TH1-polarized activated CD4⁺ T cells. YFP was expressed in MHC class I restricted thymocytes, by resting CD8⁺ T cells, and in activated CD4⁺ T cells. (J:141144)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Runx3 Mutation: 27 strains or lines available

References

• Original: J:141144 Egawa T, et al., ThPOK acts late in specification of the helper T cell lineage and suppresses Runx-mediated commitment to the cytotoxic T cell lineage. Nat Immunol. 2008 Oct;9(10):1131-9
• All: 9 reference(s)