Atg16l1^{Gt(BC0122)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Atg16l1^{Gt(BC0122)Wtsi}
• Name: autophagy related 16 like 1; gene trap BC0122, Wellcome Trust Sanger Institute
• MGI ID: MGI:3818574
• Synonyms: ATG16L1^Hm1
• Gene: Atg16l1 Location: Chr1:87683730-87720150 bp, + strand Genetic Position: Chr1, 44.44 cM
• Alliance: Atg16l1^{Gt(BC0122)Wtsi} page

Mutation origin

• Mutant Cell Line: BC0122
• Germline Transmission: Earliest citation of germline transmission: J:141419
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT2lxr
• Mutation details: A gene-trap vector containing a splice acceptor followed by a beta-geo cassette disrupted the gene locus by inserting within the intronic region after exon 6. Protein expression of both the alpha and beta isoforms of the gene product were 23-37% of wild-type as determined by immunoblots of ileal lysates from homozygous mice. This is a hypomorphic allele. (J:141419)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  BC0122	CZ259233 ; (MGI Seq Detail)	5' RACE	Chr1:87687837-87699178 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atg16l1 Mutation: 44 strains or lines available

References

• Original: J:141419 Cadwell K, et al., A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells. Nature. 2008 Nov 13;456(7219):259-63
• All: 21 reference(s)