Mcm2^{tm1(cre/ERT2)Scpr}
Targeted Allele Detail

Summary

• Symbol: Mcm2^{tm1(cre/ERT2)Scpr}
• Name: minichromosome maintenance complex component 2; targeted mutation 1, Steven C Pruitt
• MGI ID: MGI:3818719
• Synonyms: Mcm2^IRES-CreERT2, Mcm2^tm1(cre)Scpr
• Gene: Mcm2 Location: Chr6:88860456-88875762 bp, - strand Genetic Position: Chr6, 39.64 cM
• Alliance: Mcm2^{tm1(cre/ERT2)Scpr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141493
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Mcm2^{tm1(cre/ERT2)Scpr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mcm2 (MGI:105380)

• Mutation details: An IRES element, a cre/ESRI fusion gene. and a PGK-neo selection cassette were inserted into exon 16 3' to the stop codon. A cryptic polyA site 3' of the stop codon was deleted in order to permit cre expression. Cre expression was confirmed by EGFP expression after tamoxifen treatment of mice also carrying the Tg(CAG-Bgeo/GFP)21Lbe transgene. MCM2 expression was reduced to 62% of normal in heterozygotes and 35% in homozygotes. (J:141493)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mcm2 (mouse) Summary of all recombinase alleles driven by Mcm2. Comparative matrix view of recombinase activities Alliance mouse Mcm2 gene page

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Mcm2^{tm1(cre/ERT2)Scpr}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mcm2 Mutation: 51 strains or lines available

References

• Original: J:141493 Pruitt SC, et al., Reduced Mcm2 expression results in severe stem/progenitor cell deficiency and cancer. Stem Cells. 2007 Dec;25(12):3121-32
• All: 8 reference(s)