Aire^tm2Mmat
Targeted Allele Detail

Summary

• Symbol: Aire^tm2Mmat
• Name: autoimmune regulator; targeted mutation 2, Mitsuru Matsumoto
• MGI ID: MGI:3818933
• Synonyms: Aire^gfp
• Gene: Aire Location: Chr10:77865856-77879444 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Aire^tm2Mmat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141379
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Genomic sequence from immediately after the Kozak sequence in exon 1 to the exon 2 was replaced with GFP and a floxed neo cassette via homologous recombination. The neo cassette was removed via in vivo Cre mediated recombination. Immunohistochemical analysis in heterozygous mice indicates that the pattern of GFP expression is similar to the pattern of endogenous gene expression. (J:141379)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Aire Mutation: 52 strains or lines available

References

• Original: J:141379 Yano M, et al., Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance. J Exp Med. 2008 Nov 24;205(12):2827-38
• All: 8 reference(s)