Dgcr8^tm1.2Blel
Targeted Allele Detail

Summary

• Symbol: Dgcr8^tm1.2Blel
• Name: DGCR8, microprocessor complex subunit; targeted mutation 1.2, Robert Blelloch
• MGI ID: MGI:3819698
• Synonyms: Dgcr8^-, Dgcr8^delta
• Gene: Dgcr8 Location: Chr16:18071812-18107110 bp, - strand Genetic Position: Chr16, 11.31 cM, cytoband B1
• Alliance: Dgcr8^tm1.2Blel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141670
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted in intron 2 and a floxed CMV-Hygro selection cassette was inserted in intron 3. ES cells were sequentially targeted so that ES cells carried two mutated alleles. Cre recombinase treatment of these ES cells resulted in two mutant alleles, one with a floxed exon 3 but lacking the selection cassette and this allele which lacks both exon 3 and the selection cassette. (J:141670, J:161342)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dgcr8 Mutation: 67 strains or lines available

References

• Original: J:141670 Wang Y, et al., DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal. Nat Genet. 2007 Mar;39(3):380-5
• All: 13 reference(s)