Smad4^tm2.1Rob
Targeted Allele Detail

Summary

• Symbol: Smad4^tm2.1Rob
• Name: SMAD family member 4; targeted mutation 2.1, Elizabeth J Robertson
• MGI ID: MGI:3819836
• Synonyms: Smad4^deltaNES
• Gene: Smad4 Location: Chr18:73772080-73836851 bp, - strand Genetic Position: Chr18, 49.51 cM
• Alliance: Smad4^tm2.1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141589
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed PGK-hygro cassette was inserted into intron 3 and an additional loxP site was inserted into intron 4. Exon 4 and the selection cassette were removed either by transient cre expression in ES cells or by crossing chimeric mice containing the Smad4 allele to Tg(Sox2-cre)1Amc mice. (J:141589)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad4 Mutation: 48 strains or lines available

References

• Original: J:141589 Biondi CA, et al., Mice develop normally in the absence of Smad4 nucleocytoplasmic shuttling. Biochem J. 2007 Jun 1;404(2):235-45
• All: 1 reference(s)