Smad4^tm3Rob
Targeted Allele Detail

Summary

• Symbol: Smad4^tm3Rob
• Name: SMAD family member 4; targeted mutation 3, Elizabeth J Robertson
• MGI ID: MGI:3819843
• Synonyms: Smad4^MEX
• Gene: Smad4 Location: Chr18:73772080-73836851 bp, - strand Genetic Position: Chr18, 49.51 cM
• Alliance: Smad4^tm3Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141589
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Leucine 146 and Leucine 148 encoded by exon 4 were mutated to glycine and alanine respectively. A floxed PGK-hygro cassette was introduced into intron 4. Transient expression of cre recombinase subsequently removed the selection cassette from correctly targeted ES cells. (J:141589)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad4 Mutation: 48 strains or lines available

References

• Original: J:141589 Biondi CA, et al., Mice develop normally in the absence of Smad4 nucleocytoplasmic shuttling. Biochem J. 2007 Jun 1;404(2):235-45
• All: 1 reference(s)