Oprm1^tm1Jabl
Targeted Allele Detail

Summary

• Symbol: Oprm1^tm1Jabl
• Name: opioid receptor, mu 1; targeted mutation 1, Julie A Blendy
• MGI ID: MGI:3820121
• Synonyms: MOPR^A112G, Oprm1^A112G, Oprm1^A112GJabl
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^tm1Jabl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150840
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Inserted expressed sequence) Mutation: Insertion Mutation details: An A to G point mutation at position 112 was created in a BAC harboring exon 1, which corresponds to the SNP position 118 in the human sequence, and changes codon 38 from asparigine to aspartic acid (N38D). A second mutation (T108C) was introduced to abolish a BstXI restriction site without affecting the aspartic acid encoded by the codon. A floxed neomycin cassette was inserted downstream of the targeted exon and was later removed by crossing founder mice with cre transgenic mice. Protein expression in the thalamus of homozygote mice was reduced compared to mice carrying the wild-type allele. (J:150840)

Generation of the Oprm1^tm1Jabl allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:150840 Mague SD, et al., Mouse model of OPRM1 (A118G) polymorphism has sex-specific effects on drug-mediated behavior. Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10847-52
• All: 6 reference(s)