Tgfb1^tm1Rif
Targeted Allele Detail

Summary

• Symbol: Tgfb1^tm1Rif
• Name: transforming growth factor, beta 1; targeted mutation 1, Daniel B Rifkin
• MGI ID: MGI:3820285
• Synonyms: LAP^C33S, Tgfb1^C33S
• Gene: Tgfb1 Location: Chr7:25386427-25404502 bp, + strand Genetic Position: Chr7, 13.98 cM
• Alliance: Tgfb1^tm1Rif page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141786
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted
• Mutation: Single point mutation
• Mutation details: Cysteine codon 33 (TGC) was changed to serine (TCC) (p.C33S). A loxP site flanked neomycin resistance gene cassette was included in the targeting vector and was subsequently removed by crossing founder mice with cre transgenic mice. The mutated amino acid is located in the latency-associated peptide portion of the precursor protein that becomes non-covalently associated with the mature but biologically inactive (latent) form of TGF-beta. This mutation disrupts the association of the latency-associated peptide to latent TGF-beta binding proteins. Latent TGF-beta from the sera or platelets of homozygotes is not activated by shear forces. (J:141786, J:142353)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgfb1 Mutation: 35 strains or lines available

References

• Original: J:141786 Ahamed J, et al., In vitro and in vivo evidence for shear-induced activation of latent transforming growth factor-beta1. Blood. 2008 Nov 1;112(9):3650-60
• All: 5 reference(s)