Tgfb1^tm1Rif
Targeted Allele Detail

Summary

• Symbol: Tgfb1^tm1Rif
• Name: transforming growth factor, beta 1; targeted mutation 1, Daniel B Rifkin
• MGI ID: MGI:3820285
• Synonyms: LAP^C33S, Tgfb1^C33S
• Gene: Tgfb1 Location: Chr7:25386427-25404502 bp, + strand Genetic Position: Chr7, 13.98 cM
• Alliance: Tgfb1^tm1Rif page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141786
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted
• Mutation: Single point mutation
• Mutation details: These mice were generated by altering the Tgfb1 codon for C33 to one for S33 by homologous recombination. A floxed neo cassette was included in the targeting vector and was subsequently removed by crossing founder mice with cre transgenic mice. The mutated amino acid is located in the latency-associated peptide portion of the precursor protein that becomes non-covalently associated with the mature but biologically inactive (latent) form of TGF-beta. This mutation disrupts the association of the latency-associated peptide to latent TGF-beta binding proteins. Latent TGF-beta from the sera or platelets of homozygotes is not activated by shear forces. (J:141786, J:142353)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgfb1 Mutation: 35 strains or lines available

References

• Original: J:141786 Ahamed J, et al., In vitro and in vivo evidence for shear-induced activation of latent transforming growth factor-beta1. Blood. 2008 Nov 1;112(9):3650-60
• All: 5 reference(s)