Sell^tm2Tft
Targeted Allele Detail

Summary

• Symbol: Sell^tm2Tft
• Name: selectin, lymphocyte; targeted mutation 2, Thomas F Tedder
• MGI ID: MGI:3820621
• Synonyms: L(E)^neo
• Gene: Sell Location: Chr1:163889556-163908354 bp, + strand Genetic Position: Chr1, 71.37 cM
• Alliance: Sell^tm2Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142184
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The membrane-proximal region was replaced with DNA encoding a floxed neomycin resistance gene and the 7 amino acid (APVVPTR) membrane-proximal region of mouse E-selectin. It was predicted that this mutation would prevent cleavage of the protein upon cellular activation. Cell surface levels of mutated protein in homozygote blood, spleen, and lymphocytes was 27%, 34%, and 52%, respectively, of wild-type controls as determined by flow cytometry. Mutated protein was not cleaved from the cell surface upon cellular activation of lymphocytes. (J:142184)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sell Mutation: 42 strains or lines available

Notes

The name L(E)^SAME describes mice that are compound heterozygote for the Sell^tm2Tft and Sell^tm2.1Tft alleles.

References

• Original: J:142184 Venturi GM, et al., Leukocyte migration is regulated by L-selectin endoproteolytic release. Immunity. 2003 Nov;19(5):713-24
• All: 2 reference(s)