Sell^tm2.1Tft
Targeted Allele Detail

Summary

• Symbol: Sell^tm2.1Tft
• Name: selectin, lymphocyte; targeted mutation 2.1, Thomas F Tedder
• MGI ID: MGI:3820622
• Synonyms: L(E)
• Gene: Sell Location: Chr1:163889556-163908354 bp, + strand Genetic Position: Chr1, 71.37 cM
• Alliance: Sell^tm2.1Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142184
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The membrane-proximal region was replaced with DNA encoding a floxed neomycin resistance gene and the 7 amino acid (APVVPTR) membrane-proximal region of mouse E-selectin. It was predicted that this mutation would prevent cleavage of the protein upon cellular activation. Because the presence of the neo resistance gene was found to reduce expression of the gene locus in Sell^tm2Tft homozygotes, the neo gene was removed by breeding with Cre recombinase transgenic mice. Cell surface levels of mutated protein in homozygote blood, spleen, and lymphocytes was 168%, 214%, and 178%, respectively, of wild-type controls as determined by flow cytometry. Mutated protein was not cleaved from the cell surface upon cellular activation of lymphocytes. (J:142184)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sell Mutation: 42 strains or lines available

Notes

The name L(E)^SAME describes mice that are compound heterozygote for the Sell^tm2Tft and Sell^tm2.1Tft alleles.

References

• Original: J:142184 Venturi GM, et al., Leukocyte migration is regulated by L-selectin endoproteolytic release. Immunity. 2003 Nov;19(5):713-24
• All: 5 reference(s)