Ahr^tm4.1Bra
Targeted Allele Detail

Summary

• Symbol: Ahr^tm4.1Bra
• Name: aryl-hydrocarbon receptor; targeted mutation 4.1, Christopher A Bradfield
• MGI ID: MGI:3821486
• Synonyms: Ahr^dbd
• Gene: Ahr Location: Chr12:35547978-35584988 bp, - strand Genetic Position: Chr12, 15.78 cM
• Alliance: Ahr^tm4.1Bra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141978
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Six nucleotides (GGTACC), coding for amino acids glycine and serine, were inserted between the last residue of the basic domain (arginine-39) and the first residue of the helix-loop-helix domain (aspartate-40). A floxed neo selection cassette was also inserted and the removed via in vivo cre mediated recombination. Western blot analysis confirmed that the level of expression of the mutant protein in livers of homozygotes was similar to expression of the the normal protein in wild-type littermates. When expressed in vitro the mutant protein fails to bind to DNA containing dioxin responsive elements. (J:141978)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ahr Mutation: 112 strains or lines available

References

• Original: J:141978 Bunger MK, et al., Abnormal liver development and resistance to 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in mice carrying a mutation in the DNA-binding domain of the aryl hydrocarbon receptor. Toxicol Sci. 2008 Nov;106(1):83-92
• All: 6 reference(s)