Gabpb2^{Gt(RRJ488)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Gabpb2^{Gt(RRJ488)Byg}
• Name: GA repeat binding protein, beta 2; gene trap RRJ488, BayGenomics
• MGI ID: MGI:3821584
• Synonyms: GABPbeta2^-
• Gene: Gabpb2 Location: Chr3:95089077-95125227 bp, - strand Genetic Position: Chr3, 40.74 cM, cytoband F2
• Alliance: Gabpb2^{Gt(RRJ488)Byg} page

Mutation origin

• Mutant Cell Line: RRJ488
• Germline Transmission: Earliest citation of germline transmission: J:141846
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: This gene locus has three upstream non-coding exons that are alternatively spliced to create different mRNAs. This gene trap consisting of a Betageo selection/reporter cassette inserted in between the second and third non-coding exons. Immunoblot analysis of thymus and spleen extracts detected no protein signal indicating this is a null allele. (J:141846)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRJ488	CC799051 ; (MGI Seq Detail)	5' RACE	Chr3:95124972-95125043 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  26 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabpb2 Mutation: 32 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141846 Jing X, et al., GABPbeta2 is dispensible for normal lymphocyte development but moderately affects B cell responses. J Biol Chem. 2008 Sep 5;283(36):24326-33
• All: 2 reference(s)