Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis
Transgene Detail

Summary

• Symbol: Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis
• Name: transgene insertion 35-1, Yuji Mishina
• MGI ID: MGI:3821884
• Synonyms: CAG-Z-EGFP-caAlk2
• Transgene: Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis Location: unknown
• Alliance: Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACVR1 (90)

  Acvr1

  (MGI:87911)

• Mutation details: The modified chicken beta-actin promoter (CAG) was used to drive ubiquitous expression of a floxed LacZ gene with a triple polyadenylation signal sequence followed by a constitutively active human ACVR1 sequence with the amino acid substitution of glutamine with aspartic acid at position 207 (Q207D), an IRES sequence, and the EGFP coding sequence and a poly(A) signal. Five lines were established and expression analysis for one line (line 35-1) confirmed general, but not ubiquitous, expression of LacZ and cre-dependent general expression of the modified ACVR1 and EGFP. (J:109498)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: Submucous cleft palate J:233662 in mice hemizygous for Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis and Tg(KRT14-cre)1Amc.

References

• Original: J:109498 Fukuda T, et al., Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2. Genesis. 2006 Apr;44(4):159-67
• All: 24 reference(s)