Gt(ROSA)26Sor^{tm1.1(MYC/ERT2)Gev}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(MYC/ERT2)Gev}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Gerard Evan
• MGI ID: MGI:3821931
• Synonyms: MycER, R26^MER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(MYC/ERT2)Gev} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142030
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(MYC/ERT2)Gev} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MYC (4609)

  Bmyc	(MGI:88184)
  Myc	(MGI:97250)

• Mutation details: A fused human MYC and ESR1 (ERT2) sequence, preceded by a floxed neo cassette with a triple polyadenylation sequence and followed by an IRES-EGFP, was inserted into the ROSA locus. Expression of the fusion product can only be achieved through cre mediated removal of the neo cassette along with its polyadenlylation sequences. Germ line, cre mediated recombination was used to remove the neo cassette and triple polyadenylation sequences to allow for expression of the fusion gene and EGFP. (J:142030)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:142030 Murphy DJ, et al., Distinct thresholds govern Myc's biological output in vivo. Cancer Cell. 2008 Dec 9;14(6):447-57
• All: 7 reference(s)