Lepr^tm1Yli
Targeted Allele Detail

Summary

• Symbol: Lepr^tm1Yli
• Name: leptin receptor; targeted mutation 1, Yong Liu
• MGI ID: MGI:3822311
• Synonyms: Ob-Rb^{F985/1077/1138}, Y123F
• Gene: Lepr Location: Chr4:101574601-101672549 bp, + strand Genetic Position: Chr4, 46.96 cM
• Alliance: Lepr^tm1Yli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142218
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Three tyrosine residues in exon 18 (985, 1077, 1138) were replaced with phenylalanine and a floxed neo cassette was inserted after exon 18 via homologous recombination. The floxed neo cassette was then removed by cre mediated recombination leaving behind a single loxP site. Quantitative RT-PCR indicated that expression of the long isoform in the hypothalamus of homozygotes was similar to that in wild-type mice. Replacement of these tyrosines with phenylalanines abolished leptin stimulated STAT3 phosphorylation in the hypothalamus. (J:142218)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lepr Mutation: 122 strains or lines available

References

• Original: J:142218 Jiang L, et al., Tyrosine-dependent and -independent actions of leptin receptor in control of energy balance and glucose homeostasis. Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18619-24
• All: 3 reference(s)