Foxa2^tm1(cre)Heli
Targeted Allele Detail

Summary

• Symbol: Foxa2^tm1(cre)Heli
• Name: forkhead box A2; targeted mutation 1, Heiko Lickert
• MGI ID: MGI:3822329
• Synonyms: Foxa2^iCre, Foxa2^iCredeltaneo, Foxa2^tm1(cre)Lic
• Gene: Foxa2 Location: Chr2:147884797-147888889 bp, - strand Genetic Position: Chr2, 73.38 cM
• Alliance: Foxa2^tm1(cre)Heli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142568
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Hypomorph, Recombinase)
• Mutation: Insertion
• Foxa2^tm1(cre)Heli expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxa2 (MGI:1347476)

• Mutation details: A mini gene locus including a cDNA encoding a codon improved cre, a translational stop signal, an artificial intron and an SV40 polyadenylation signal , and an frt flanked neo cassette in reverse orientation were inserted into exon 1 via homologous recombination. The neo cassette was then removed by in vivo Flp mediated recombination. Western blot analysis of liver lysates from homozygous mice indicated protein expression at about 10% to 15% that of controls. Cre-reporter activity analysis indicates functional Cre is found in endoderm precursor cells. (J:142568)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxa2 (mouse) Summary of all recombinase alleles driven by Foxa2. Comparative matrix view of recombinase activities Alliance mouse Foxa2 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxa2 Mutation: 28 strains or lines available

References

• Original: J:142568 Uetzmann L, et al., A mouse line expressing Foxa2-driven Cre recombinase in node, notochord, floorplate, and endoderm. Genesis. 2008 Oct;46(10):515-22
• All: 9 reference(s)