Plk1^{Gt(RRR358)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Plk1^{Gt(RRR358)Byg}
• Name: polo like kinase 1; gene trap RRR358, BayGenomics
• MGI ID: MGI:3822515
• Gene: Plk1 Location: Chr7:121758662-121769096 bp, + strand Genetic Position: Chr7, 65.52 cM
• Alliance: Plk1^{Gt(RRR358)Byg} page

Mutation origin

• Mutant Cell Line: RRR358
• Germline Transmission: Earliest citation of germline transmission: J:142593
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT0Lxf was inserted after exon 9. Western blot analysis failed to detect truncated or fusion protein products in tissues from heterozygous mice. (J:142593)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR358	CG784800 ; (MGI Seq Detail)	5' RACE	Chr7:121768087-121768383 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plk1 Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:142593 Lu LY, et al., Polo-like kinase 1 is essential for early embryonic development and tumor suppression. Mol Cell Biol. 2008 Nov;28(22):6870-6
• All: 2 reference(s)