Bcam^tm1Jhm
Targeted Allele Detail

Summary

• Symbol: Bcam^tm1Jhm
• Name: basal cell adhesion molecule; targeted mutation 1, Jeffrey H Miner
• MGI ID: MGI:3822736
• Gene: Bcam Location: Chr7:19490063-19504457 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Bcam^tm1Jhm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142595
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 2 kb fragment containing exons 11 though 13 was deleted resulting in the absence of 142 amino acids of the fifth Ig domain, the transmembrane domain, and the first 20 amino acids of the cytoplasmic tail. Splicing from exon 10 to 14 results in an out of frame transcript. The absence of protein exppression was confirmed by immunohistochemistry on neuromuscular junctions. (J:142595)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcam Mutation: 35 strains or lines available

References

• Original: J:142595 Nishimune H, et al., Laminins promote postsynaptic maturation by an autocrine mechanism at the neuromuscular junction. J Cell Biol. 2008 Sep 22;182(6):1201-15
• All: 2 reference(s)