Ezh2^tm2Sho
Targeted Allele Detail

Summary

• Symbol: Ezh2^tm2Sho
• Name: enhancer of zeste 2 polycomb repressive complex 2 subunit; targeted mutation 2, Stuart Orkin
• MGI ID: MGI:3823217
• Synonyms: Ezh2^fl, Ezh2^flox
• Gene: Ezh2 Location: Chr6:47507073-47572275 bp, - strand Genetic Position: Chr6, 22.92 cM, cytoband B
• Alliance: Ezh2^tm2Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142193
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neo cassette was inserted into intron 13 and an additional loxP site was inserted into intron 15. Transient cre expression was used to remove the neo cassette in ES cells leaving exons 14 and 15 floxed. (J:142193)

Disease models

Expression

In Mice Carrying this Mutation:	14 assay results 8 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ezh2 Mutation: 72 strains or lines available

References

• Original: J:142193 Shen X, et al., EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol Cell. 2008 Nov 21;32(4):491-502
• All: 65 reference(s)