Tg(CAG-OTC/CAT)#Wcl
Transgene Detail

Summary

• Symbol: Tg(CAG-OTC/CAT)#Wcl
• Name: transgene insertion, Warren C Ladiges
• MGI ID: MGI:3823244
• Synonyms: MCAT, Tg(CAT)#Wcl
• Transgene: Tg(CAG-OTC/CAT)#Wcl Location: unknown
• Alliance: Tg(CAG-OTC/CAT)#Wcl page

Transgene origin

• Strain of Origin: C57BL/6 x (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: To generate the transgene, the 11 carboxy-terminal amino acids, including the peroxisomal localization signal, were deleted from the human catalase gene. The initiating methionine was also deleted and the first 25 amino acids of the human ornithine transcarbamylase leader sequence were added to the amino terminus to target this protein product to mitochondria. The catalase cDNA construct was then cloned into a plasmid under the influence of the chicken-actin promoter/CMV enhancer (CAG). The catalase activity in the cardiac mitochondrial fraction of transgenic animals is 50 times higher than that in their wild-type littermates. This record represents pooled data for multiple lines. (J:117831)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CAG-OTC/CAT)#Wcl

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:142955 Treuting PM, et al., Reduction of age-associated pathology in old mice by overexpression of catalase in mitochondria. J Gerontol A Biol Sci Med Sci. 2008 Aug;63(8):813-22
• All: 11 reference(s)