Lama5^tm3Jhm
Targeted Allele Detail

Summary

• Symbol: Lama5^tm3Jhm
• Name: laminin, alpha 5; targeted mutation 3, Jeffrey H Miner
• MGI ID: MGI:3823246
• Synonyms: Lama5^neo
• Gene: Lama5 Location: Chr2:179818166-179867652 bp, - strand Genetic Position: Chr2, 102.7 cM
• Alliance: Lama5^tm3Jhm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104565
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to flox exons 15-21, which encodes 40% of domain V that consists of laminin EGF-like repeats. Unlike Lama5^tm2Jhm, the frt-flanked neo cassette inserted downstream of exon 21 was retained and leads to an alternate transcript involving the splicing of exon 20 to exon 22. (J:128225)

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lama5 Mutation: 153 strains or lines available

References

• Original: J:104565 Nguyen NM, et al., Epithelial laminin alpha5 is necessary for distal epithelial cell maturation, VEGF production, and alveolization in the developing murine lung. Dev Biol. 2005 Jun 1;282(1):111-25
• All: 3 reference(s)