Arg1^tm1Pmu
Targeted Allele Detail

Summary

• Symbol: Arg1^tm1Pmu
• Name: arginase, liver; targeted mutation 1, Peter J Murray
• MGI ID: MGI:3826847
• Synonyms: Arg1^flox, Arg^fl
• Gene: Arg1 Location: Chr10:24791105-24803368 bp, - strand Genetic Position: Chr10, 12.26 cM
• Alliance: Arg1^tm1Pmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143229
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 7 and a loxP-flanked neomycin selection cassette was inserted downstream of exon 8. Transient expression of cre recombinase removed the neomycin cassette, leaving exons 7 and 8 floxed. These exons encode in addition to the oligomerization domain, two highly conserved aspartic acid residues that are essential to the enzymatic activity of the gene product. Correct targeting was confirmed by genomic PCR. (J:143229)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Arg1 Mutation: 28 strains or lines available

References

• Original: J:143229 El Kasmi KC, et al., Toll-like receptor-induced arginase 1 in macrophages thwarts effective immunity against intracellular pathogens. Nat Immunol. 2008 Dec;9(12):1399-406
• All: 57 reference(s)