Gt(ROSA)26Sor^tm3(Gli3)Amc
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm3(Gli3)Amc
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Andrew P McMahon
• MGI ID: MGI:3828280
• Synonyms: Gli3T^Flagc, R26-Gli3T, RosaGli3TFlag
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm3(Gli3)Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143454
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 and C57BL/6 and CBA and Swiss Webster

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^tm3(Gli3)Amc expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gli3 (MGI:95729)

• Mutation details: A loxP site flanked neomycin resistance gene cassette, a cDNA encoding the first 740 amino acids of Gli3, three copies of the sequence coding for a flag tag, and an IRES-YFP (Venus) cassette were inserted into the locus. Expression is only achieved following cre-mediated removal of the floxed neo cassette. (J:143454)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

ES cell line = YFP3.1, a male ES cell line carrying Tg(CAG-cre/Esr1*)5Amc and is compound heterozgous at Gt(ROSA)26Sor: Gt(ROSA)26Sor^tm1Sor and Gt(ROSA)26Sor^tm1(EYFP)Cos.

References

• Original: J:143454 Vokes SA, et al., A genome-scale analysis of the cis-regulatory circuitry underlying sonic hedgehog-mediated patterning of the mammalian limb. Genes Dev. 2008 Oct 1;22(19):2651-63
• All: 13 reference(s)