Tg(KRT5-cre/ERT)ICmch
Transgene Detail

Summary

• Symbol: Tg(KRT5-cre/ERT)ICmch
• Name: transgene insertion I, Chun-Ming Chen
• MGI ID: MGI:3829358
• Synonyms: K5-CreER, Tg(BK5-CreERT)I
• Transgene: Tg(KRT5-cre/ERT)ICmch Location: unknown
• Alliance: Tg(KRT5-cre/ERT)ICmch page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(KRT5-cre/ERT)ICmch expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  cattle	KRT5 (281268)

• Mutation details: This transgene comprises 5.2 kb of 5' flanking and promoter DNA from the bovine keratin 5 gene and a rabbit beta-globin intron followed by the Cre recombinase coding sequence joined in-frame to a mutated estrogen receptor ligand-binding domain (ER^T) that recognizes 4-hydroxytamoxifen (4-OHT), but not endogenous estrogen (beta-17 estradiol), then by an SV40 poly(A) signal and two reversed copies of the ~1.2 kb HS4 5' insulator sequence from the chicken beta-globin gene. X-gal staining of tissues from 4-OHT treated mice from crosses of females with the transgene and males bearing the Cre reporter, Gt(ROSA)26Sor, demonstrated expression in all tissues expressing keratin 5 -- generally, in stratified and pseudo-stratified epithelia throughout the body. (J:143780)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	KRT5 (cattle) Summary of all recombinase alleles driven by KRT5. Comparative matrix view of recombinase activities

Tumor Data

List all tumor models in MMHCdb carrying Tg(KRT5-cre/ERT)ICmch

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:143780 Liang CC, et al., Transgenic mice exhibiting inducible and spontaneous Cre activities driven by a bovine keratin 5 promoter that can be used for the conditional analysis of basal epithelial cells in multiple organs. J Biomed Sci. 2009;16:2
• All: 8 reference(s)