Tg(KRT5-cre/ERT)SCmch
Transgene Detail

Summary

• Symbol: Tg(KRT5-cre/ERT)SCmch
• Name: transgene insertion S, Chun-Ming Chen
• MGI ID: MGI:3829360
• Synonyms: spontaneous BK5-CreERT transgene, Tg(BK5-CreERT)S
• Transgene: Tg(KRT5-cre/ERT)SCmch Location: unknown
• Alliance: Tg(KRT5-cre/ERT)SCmch page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(KRT5-cre/ERT)SCmch expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  cattle	KRT5 (281268)

• Mutation details: This transgene comprises 5.2 kb of 5' flanking and promoter DNA from the bovine keratin 5 gene and a rabbit beta-globin intron followed by the Cre recombinase coding sequence joined in-frame to a mutated estrogen receptor ligand binding domain (ER^T), then by an SV40 poly(A) signal and two reversed copies of the ~1.2 kb HS4 5' insulator sequence from the chicken beta-globin gene. Double transgenic mice for cre and the R26-LacZ reporter demonstrate constitive expression (in absence of 4-hydroxy tamoxifen treatment) from about E14.5 in most keratin 5 expressing tissues; the thymic epithelium and the Mullerian vagina show significant expression only after postnatal day 7. Additionally, only this line (in contrast with Tg(KRT5-Cre/ERT)ICmch) exhibits Cre recombinase activity in KRT5-expressing epithelial cells of the mammary gland. (J:143780)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	KRT5 (cattle) Summary of all recombinase alleles driven by KRT5. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:143780 Liang CC, et al., Transgenic mice exhibiting inducible and spontaneous Cre activities driven by a bovine keratin 5 promoter that can be used for the conditional analysis of basal epithelial cells in multiple organs. J Biomed Sci. 2009;16:2
• All: 2 reference(s)