Glrx3^{Gt(RRF094)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Glrx3^{Gt(RRF094)Byg}
• Name: glutaredoxin 3; gene trap RRF094, BayGenomics
• MGI ID: MGI:3829374
• Synonyms: PICOT^-
• Gene: Glrx3 Location: Chr7:137039343-137070323 bp, + strand Genetic Position: Chr7, 82.32 cM, cytoband F5
• Alliance: Glrx3^{Gt(RRF094)Byg} page

Mutation origin

• Mutant Cell Line: RRF094
• Germline Transmission: Earliest citation of germline transmission: J:143577
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The pGT0 gene trap vector, which contains a beta-geo cassette, was inserted into intron 2. A 50% reduction in protein expression in heterozygous samples was confirmed by western blot analysis. (J:143577)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRF094	CC249286 ; (MGI Seq Detail)	5' RACE	Chr7:137039377-137046813 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Glrx3 Mutation: 33 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:143577 Cha H, et al., PICOT is a critical regulator of cardiac hypertrophy and cardiomyocyte contractility. J Mol Cell Cardiol. 2008 Dec;45(6):796-803
• All: 4 reference(s)