Lrig3^{Gt(LST016)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Lrig3^{Gt(LST016)Byg}
• Name: leucine-rich repeats and immunoglobulin-like domains 3; gene trap LST016, BayGenomics
• MGI ID: MGI:3829384
• Gene: Lrig3 Location: Chr10:125802088-125851228 bp, + strand Genetic Position: Chr10, 73.66 cM, cytoband D3
• Alliance: Lrig3^{Gt(LST016)Byg} page

Mutation origin

• Mutant Cell Line: LST016
• Germline Transmission: Earliest citation of germline transmission: J:142510
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2TMpfs Vector Type: secretory trap
• Mutation details: The pGT2TMPFS gene trap vector containing a beta-geo cassette was inserted downstream of exon 2. Transcript expression is reduced to 3% of wild-type as determined by quantitative RT-PCR. This allele is severely hyppomorphic. (J:67920, J:142510)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  LST016	CC144591 ; (MGI Seq Detail)	5' RACE	Chr10:125808024-125808335 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lrig3 Mutation: 64 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:67920 Leighton PA, et al., Defining brain wiring patterns and mechanisms through gene trapping in mice. Nature. 2001 Mar 8;410(6825):174-9
• All: 3 reference(s)