Pthlh^tm1Broa
Targeted Allele Detail

Summary

• Symbol: Pthlh^tm1Broa
• Name: parathyroid hormone-like peptide; targeted mutation 1, Arthur E Broadus
• MGI ID: MGI:3829430
• Synonyms: PTHrP-lacZ
• Gene: Pthlh Location: Chr6:147153607-147165511 bp, - strand Genetic Position: Chr6, 78.19 cM, cytoband F-G
• Alliance: Pthlh^tm1Broa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128121
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was replaced with a LacZ reporter cassette. The lacZ cassette encoded the beta-gal coding region preceded by stop codons (TAG) in all three reading frames. The construct also uses a polyA sequence to replace the untranslated region of the gene locus, which contains AU-rich instability sequences. Beta-gal activity was detected in numerous tissues of heterozygous embryos including the mammary bud, inner ear, nasal epithelium, and in the endochondral skeleton. Beta-gal activity was also observed in tendon insertion sites of adult mice and within descrete populations of chondrocytes. (J:128121)

Expression

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pthlh Mutation: 19 strains or lines available

References

• Original: J:128121 Chen X, et al., Initial characterization of PTH-related protein gene-driven lacZ expression in the mouse. J Bone Miner Res. 2006 Jan;21(1):113-23
• All: 15 reference(s)