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Tcrbtm5Slek
Targeted Allele Detail
Summary
Symbol: Tcrbtm5Slek
Name: T cell receptor beta chain; targeted mutation 5, Barry P Sleckman
MGI ID: MGI:3829440
Synonyms: Jbeta1sigma
Gene: Tcrb  Location: Chr6:40868230-41535305 bp, + strand  Genetic Position: Chr6, cytoband A-C
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:143738
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsES cells containing Tcrbtm1Slek, which contain a loxP site in place of the Dbeta2-Jbeta2 gene cluster, were additionally targeted to generate a TCRb allele with only a single Dbeta and Jbeta gene segment. Five of the six Jbeta1 segments (Jbeta1.2-1.6) were replaced with a loxP-flanked neomycin cassette. The neomycin resistance gene was then removed by cre-mediated recombination. This allele thus has a 2 kb deletion of the Dbeta2-Jbeta2 gene segment cluster, and a 1.5 kb deletion of five Jbeta1 gene segments. As a result, all TCRbeta chain genes assembled on this allele use the Dbeta1 and Jbeta1.1 gene segments. (J:143738)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tcrb Mutation:  97 strains or lines available
References
Original:  J:143738 Khor B, et al., Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRbeta locus. Mol Immunol. 2009 Jan;46(3):321-6
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory