Tcrbtm5Slek
Targeted Allele Detail
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Symbol: |
Tcrbtm5Slek |
Name: |
T cell receptor beta chain; targeted mutation 5, Barry P Sleckman |
MGI ID: |
MGI:3829440 |
Synonyms: |
Jbeta1sigma |
Gene: |
Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C
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Germline Transmission: |
Earliest citation of germline transmission:
J:143738
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intergenic deletion
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Mutation details: ES cells containing Tcrbtm1Slek, which contain a loxP site in place of the Dbeta2-Jbeta2 gene cluster, were additionally targeted to generate a TCRb allele with only a single Dbeta and Jbeta gene segment. Five of the six Jbeta1 segments (Jbeta1.2-1.6) were replaced with a loxP-flanked neomycin cassette. The neomycin resistance gene was then removed by cre-mediated recombination. This allele thus has a 2 kb deletion of the Dbeta2-Jbeta2 gene segment cluster, and a 1.5 kb deletion of five Jbeta1 gene segments. As a result, all TCRbeta chain genes assembled on this allele use the Dbeta1 and Jbeta1.1 gene segments.
(J:143738)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Tcrb Mutation: |
97 strains or lines available
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Original: |
J:143738 Khor B, et al., Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRbeta locus. Mol Immunol. 2009 Jan;46(3):321-6 |
All: |
1 reference(s) |
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