Tcrb^tm6Slek
Targeted Allele Detail

Summary

• Symbol: Tcrb^tm6Slek
• Name: T cell receptor beta chain; targeted mutation 6, Barry P Sleckman
• MGI ID: MGI:3829441
• Synonyms: Jbeta1^sigma.1
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143738
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Intragenic deletion, Nucleotide substitutions
• Mutation details: ES cells containing Tcrb^tm1Slek, which contain a loxP site in place of the Dbeta2-Jbeta2 gene cluster, were additionally targeted to generate a TCRb allele with only a single wild-type Dbeta gene segment and a single mutated Jbeta gene segment. Five of the six Jbeta1 segments (Jbeta1.2-1.6) were replaced with a loxP-flanked neomycin cassette. The neomycin resistance gene was then removed by cre-mediated recombination. In addition, the Jbeta1.1 heptamer-nonamer recombination sequence motif (cacagtg-gagaaaaat) was mutated to "ttttaaa-ccctctccc" to disrupt Rag mediated recombination. This allele thus has a 2 kb deletion of the Dbeta2-Jbeta2 gene segment cluster, and a 1.5 kb deletion of five Jbeta1 gene segments. As a result, all TCRbeta chain genes assembled on this allele use the Dbeta1 and the mutated Jbeta1.1 gene segments. (J:143738)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:143738 Khor B, et al., Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRbeta locus. Mol Immunol. 2009 Jan;46(3):321-6
• All: 1 reference(s)