Sohlh2^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Sohlh2^tm1Miya
• Name: spermatogenesis and oogenesis specific basic helix-loop-helix 2; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:3829597
• Gene: Sohlh2 Location: Chr3:55089465-55117378 bp, + strand Genetic Position: Chr3, 26.53 cM
• Alliance: Sohlh2^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143535
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 8 were replaced with an IRES beta-geo cassette and puromycin selection marker. The absence of protein expression was confirmed by western blot analysis on testes extracts. (J:143535)

Expression

In Mice Carrying this Mutation:	49 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sohlh2 Mutation: 27 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:143535 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:143535 Toyoda S, et al., Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol. 2009 Jan 1;325(1):238-48
• All: 1 reference(s)