Igk^tm1Mss
Targeted Allele Detail

Summary

• Symbol: Igk^tm1Mss
• Name: immunoglobulin kappa chain complex; targeted mutation 1, Mark S Schlissel
• MGI ID: MGI:3829662
• Synonyms: Ckappa-iYFP
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143876
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

Allele Type: Targeted (Reporter) Mutation: Insertion Mutation details: A targeting vector was created by cloning a floxed neomycin resistance gene followed by an IRES-YFP cassette into a BsmBI site located 9 bp downstream of the Igk-C stop codon. This construct was introduced into ES cells containing a Prm1-cre transgene and male chimeric mice were generated. Expression of the cre transgene in male germline cells removed the neo cassette. The Prm1-cre transgene was bred out of the resulting mice. YFP expression was observed in most pre-, immature-, and mature B cells of heterozygote mice. (J:143876)

Generation of the Igk^tm1Mss allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 26 strains or lines available

Notes

ES cells contain a Prm1-cre transgene.

References

• Original: J:143876 Amin RH, et al., Biallelic, ubiquitous transcription from the distal germline Ig{kappa} locus promoter during B cell development. Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):522-7
• All: 1 reference(s)