Spic^tm1.1Kmm
Targeted Allele Detail

Summary

• Symbol: Spic^tm1.1Kmm
• Name: Spi-C transcription factor (Spi-1/PU.1 related); targeted mutation 1.1, Kenneth M Murphy
• MGI ID: MGI:3829865
• Synonyms: Spi-C^null
• Gene: Spic Location: Chr10:88511131-88518885 bp, - strand Genetic Position: Chr10, 44.14 cM, cytoband C
• Alliance: Spic^tm1.1Kmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143900
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-5 were replaced with a floxed neomycin cassette. Founder mice were crossed with Tg(CMV-cre)1Cgn/J transgenic mice to remove the floxed neo cassette, leaving behind a single loxP site. Correct targeting and removal of the neo cassette was confirmed by Southern blot. (J:143900)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spic Mutation: 24 strains or lines available

Notes

ES cell line = MC50 or EDJ22 (129S6/SvEvTac).

References

• Original: J:143900 Kohyama M, et al., Role for Spi-C in the development of red pulp macrophages and splenic iron homeostasis. Nature. 2009 Jan 15;457(7227):318-21
• All: 8 reference(s)