Inpp5d^tm1Wgk
Targeted Allele Detail

Summary

• Symbol: Inpp5d^tm1Wgk
• Name: inositol polyphosphate-5-phosphatase D; targeted mutation 1, William G Kerr
• MGI ID: MGI:3830362
• Synonyms: SHIP^flox
• Gene: Inpp5d Location: Chr1:87548034-87648229 bp, + strand Genetic Position: Chr1, 44.44 cM, cytoband C5
• Alliance: Inpp5d^tm1Wgk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95215
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and a floxed neomycin cassette was placed downstream of exon 1. Cre recombinase was transiently expressed in correctly targeted ES cells to remove the neomycin selection cassette, leaving exon 1 flanked by loxP sites. Genomic PCR and Southern blotting confirmed removal of the neo cassette and the flox-state of exon 1. (J:95215, J:144110)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Inpp5d Mutation: 94 strains or lines available

References

• Original: J:95215 Wang JW, et al., Influence of SHIP on the NK repertoire and allogeneic bone marrow transplantation. Science. 2002 Mar 15;295(5562):2094-7
• All: 21 reference(s)